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FPLC purification of allurin. (A) Elution profile of 12HEW applied to a FPLC anion exchange column and eluted with 50 mM phosphate buffer (pH 7.45) and a linear gradient of NaCl. (B) Sperm chemotaxis activity. Sperm chemotaxis assays of each FPLC fraction indicate that high activity is found in fraction 3 with lower activity found in fraction 7. (C) SDS/PAGE analysis of each of the numbered fractions in A. Allurin is seen as a major band at Mr = 23 kDa (arrows) in fractions 3 and 7.
