Fig. 3.
Competition assay in the presence or absence of ORF 2 peptide. A mixture of wild type strain 110.58 and its mutant ΔORF2 was incubated in (a) the presence of 0.07 mg/ml of ORF2 ligand peptide FPPQSV or (b) the absence of peptide. At the start and at OD600nm = 0.2 and 0.3 CFU/ml were determined to show that the wild type has a competitive advantage and that this is greater in the presence of the peptide. ****p < 0.0001, *p = 0.015. Results are the mean of three independent experiments, error bars show the standard error