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. 2018 Mar 27;18:23. doi: 10.1186/s12866-018-1167-y

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effect of wild type pneumococcal strain 110.58 and its mutants ΔORF 1 + 2 and ΔORF 2 on CXCL8 induction in human nasopharyngeal Detroit 562 epithelial cells. ORF2 ligand peptide FPPQSV was either not added (open bars) or added to give a final a concentration of 0.07 mg/ml (shaded bars). No significant differences were observed between the strains under the same conditions. The bars indicate the mean of three independent experiments and error bars indicate standard error. Negative control refers to Detroit cells in the absence of bacteria