Table 2.
Characterization of organoids and cell lines
| Tissue Bank # | Source | 2D Culture | Days to Split | KRAS mutation | PDX1 | CK19 | Maspin | CRR9 | αSMA | Vimentin |
|---|---|---|---|---|---|---|---|---|---|---|
| 2112 | primary | N | 7 | + | + | |||||
| 1914 | liver met | N | 7 | + | + | |||||
| 2220 | primary | N | 3 | + | +/− | |||||
| 2423 | lung met | Y | 5 | G12 V | + | +(+) | +(2.7) | +(3) | -(−) | – |
| 1710 | ascites | Y | 1 | G12D | – | + | + | + | – | – |
| RA012 | rapid autopsy | Y | 27 | WT | + | + | -(−) | + | – | – |
| RA012 met | rap. Aut. met | N | 20 | WT | + | + | + | – | – | |
| 2534 | omentum | N | 3 | G12D | + | +(+) | +(2.7) | +(3) | -(−) | – |
| 2475 | liver met | N | 17 | WT | – | +(+) | +(1.0) | +(3) | -(−) | – |
| 2535 | peritoneum | N | 5 | G12 V | + | +(+) | (2.7) | +(3) | -(−) | – |
| 2423CAF | CAFs | Y | WT | – | – | +*(+) | + | |||
| RA012CAF | CAFs | Y | WT | – | – | +* | + |
normal adj. = tumor adjacent normal tissue. Rap. aut. Met = metastatic tumor from rapid autopsy. Blank – not determined. *αSMA was positive is a subpopulation in co-culture with organoids. Tumor tissue staining indices are in parentheses (IHC: 1 = weak, 2 = moderate, 3 = strong, multiplied by percentage of positive cells)