Fig. 5.

Deeper sequencing of four primer sets yields greater coverage of M. tuberculosis genome. The colored lines indicate individual replicates and the green dashed line is the pooled total. All four sets yield ∼10-fold increases in efficiency over the unamplified samples (black dashed line). The primer sets reach ×10 coverage on between 28 and 50% of the target genome while the unamplified controls were at < 2.5% ×10 coverage with 1.5 Gbp of sequencing