Table 2.
Characteristics of primer sets chosen for selective whole-genome amplification of M. tuberculosis from human DNA, ordered by ratio
| Ratio | # Primers | Gini | Mean target dist. | Mean bg. dist. | |
|---|---|---|---|---|---|
| Mtb6a | 0.0057 | 7 | 0.501 | 1.95E+03 | 3.41E+05 |
| Mtb9a | 0.0058 | 7 | 0.538 | 1.78E+03 | 3.05E+05 |
| Mtb4a | 0.0062 | 7 | 0.512 | 1.88E+03 | 3.04E+05 |
| Mtb8a | 0.0062 | 7 | 0.533 | 1.80E+03 | 2.92E+05 |
| Mtb7 | 0.0066 | 7 | 0.499 | 2.03E+03 | 3.09E+05 |
| Mtb2 | 0.0095 | 7 | 0.484 | 3.29E+03 | 3.45E+05 |
| Mtb5 | 0.0155 | 6 | 0.480 | 5.00E+03 | 3.22E+05 |
| Mtb1 | 0.0171 | 7 | 0.476 | 4.97E+03 | 2.90E+05 |
| Mtb3 | 0.0172 | 7 | 0.478 | 4.99E+03 | 2.90E+05 |
| Mtb10 | 0.0181 | 7 | 0.479 | 4.29E+03 | 2.37E+05 |
| MtbUneven | 0.0140 | 2 | 0.623 | 1.14E+04 | 8.10E+05 |
| MtbSparse | 0.0387 | 3 | 0.505 | 2.60E+04 | 6.71E+05 |
‘Ratio’ indicates the ratio between the average distance between binding distances in the target and background. Primer sequences are listed in Supplementary Table 2.
a
The sets that most effectively amplified Mycobacterium for SWGA.