Extended Data Figure 7. SFB-specific 7B8 Tg CD4⁺ T cells produce IL-17a upon transfer to MIA-exposed pregnant mothers.

a, Schematic of the experimental design. b-c, Both TCRα KO and IL-17a KO females, with or without adoptive transfers of 7B8 Tg-derived CD4⁺ T cells, were crossed with B6 WT males to produce heterozygous WT offspring. USV index (n=16/30 (TCRα KO; poly(I:C)/7B8 Tg T cell transfer); n=23/23 (IL-17a KO;poly(I:C)/7B8 Tg T cell transfer), marble burying index, time spent in the center of an open field, and % interaction and total distance traveled during the sociability test of TCRα KO (b) or IL-17a KO (c) offspring (n=12/15 (TCRα KO; poly(I:C)/7B8 Tg T cell transfer); n=12/14 (IL-17a KO;poly(I:C)/7B8 Tg T cell transfer). Data pooled from 2-3 independent experiments. d-e, Representative SATB2 staining in the cortex of the animals prepared as in (a). Arrows indicate cortical patches. Scale bar, 100 μm. f-g, Quantification of SATB2⁺ cells (n=7/6 (TCRα KO;poly(I:C)/7B8 Tg T cell transfer); n=6/7 (IL-17a KO;poly(I:C)/7B8 Tg T cell transfer). h, Cortical patch size (n=5/5 (TCRα KO;poly(I:C)/7B8 Tg T cell transfer); n=4/4 (IL-17a KO;poly(I:C)/7B8 Tg T cell transfer). i-j, IL-17a concentrations in maternal plasma collected at E14.5. k, Flow cytometry of ileal CD4⁺ T cells (gated on CD4⁺TCR-β⁺) stained intracellularly for IL-17a. Mononuclear cells were collected from small intestines of poly(I:C)-treated IL-17a KO mothers transferred with 7B8 Tg CD4⁺ T cells. CD45.1⁺ cells refer to donor cells and CD45.2⁺ to recipient cells. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 as calculated by Student’s t-test (b-c,h-j) and one-way (f-g) ANOVA with Sidak post-hoc tests. Graphs indicate mean +/− s.e.m.