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. Author manuscript; available in PMC: 2018 Mar 28.
Published in final edited form as: Nature. 2017 Sep 13;549(7673):528–532. doi: 10.1038/nature23910

Extended Data Figure 8. A mix of twenty human commensals induces colonic Th17 cell differentiation in SFB-absent Jax mice.

Extended Data Figure 8

a, Schematic of the experimental design. b, Flow cytometry of CD4+ T cells (gated on CD4+TCR-β+) stained intracellularly for IL-17a and RORγt. Mononuclear cells were collected from colons of poly(I:C)-treated Jax mothers with/without human bacteria-gavage. c, Representative SEM images of epithelial surfaces in the ilea from 2 independent experiments. d-e, Total interaction time (d), and total distance traveled (e) during the sociability test of adult offspring described in (a) (n=23/22/13 for vehicle-gavaged only/human bacteria-gavaged+isotype control antibody/human bacteria-gavaged+anti-IL-17a antibody; 4 independent experiments). f-g, Quantification of SATB2+ cells (n=5/group) and cortical patch size (n=7/6/5 (poly(I:C);vehicle-treated Jax/human bacteria-gavaged Jax with isotype control antibody/human bacteria-gavaged Jax with anti-IL-17a antibody). *p<0.05 as calculated by one-way (d, e, g) and two-way (f) ANOVA with Tukey post-hoc test. Graphs indicate mean +/− s.e.m.