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. Author manuscript; available in PMC: 2018 Oct 3.
Published in final edited form as: Environ Sci Technol. 2017 Sep 22;51(19):11115–11125. doi: 10.1021/acs.est.7b03180

Figure 4.

Figure 4

Effect of Fe(II) on ArsI protein fluorescence. Fe(II)-dependent quenching intrinsic protein fluorescence of wild type ArsI and derivatives. Fluorescence was assayed at 23 °C with excitation and emission wavelengths of 295 and 340 nm, respectively, as described in Materials and Methods. (NH4)2Fe(SO4)2·6H2O prepared in degassed buffer (25 μM, final concentration) was added to 5 μM ArsI at indicated time (arrow).