Fig. 4.

KPNA7 is vital for the maintenance of proper nuclear morphology. a Hs700T pancreatic cancer cells and (c) T-47D breast cancer cells were treated with control (LUC) or KPNA7 siRNA and stained with lamin A/C antibody (green) 96 h post-transfection. The nuclei were counterstained with DAPI (blue). White squares indicate an individual cell for which an enlarged image is shown and white vertical lines pinpoint the location of which a cross-section of the nucleus is illustrated. The percentages of normal vs. aberrant nuclei were determined for (b) Hs700T and (d) T-47D cells from a minimum of six microscopic images. The mean and SD of the replicates are shown. e, f The nuclear area, XY aspect ratio and YZ aspect ratio were determined from 50 nuclei using the ImageJ software. The median is indicated with a horizontal line. **p < 0.01, ***p < 0.005