Table 1. Primer-probe sequences for Q-RT-PCR, PCR and sequencing primer sequences for pyrosequencing (Btn: biotin) and qPCR primer sequences for ChIP.
cDNA primers | Sequence |
---|---|
hprt | |
Forward | CAC TGG CAA AAC AAT GCA GAC T |
Reverse | GTC TGG CTT ATA TCC AAC ACT TCG T |
Probe | CAA GCT TGC GAC CTT GAC CAT CTT TGG A |
sst1 | |
Forward | CAC CGT GGC CAA GGT AGT AAA |
Reverse | CCA CGA TGG GCA GGA TGA |
Probe | CTG GGC GTG TGG GTG CTA TCG C |
sst2 | |
Forward | TCG GCC AAG TGG AGG AGA C |
Reverse | AGA GAC TCC CCA CAC AGC CA |
Probe | CCG GAC GGC CAA GAT GAT CAC C |
sst3 | |
Forward | CTG GGT AAC TCG CTG GTC ATC TA |
Reverse | AGC GCC AGG TTG AGG CTG TA |
Probe | CGG CCA GCC CTT CAG TCA CCA AC |
sst5 | |
Forward | CAT CCT CTC CTA CGC CAA CAG |
Reverse | GGA AGC TCT GGC GGA AGT T |
Probe | CCC GTC CTC TAC GGC TTC CTC TCT GA |
Pyrosequencing primers | sequence |
---|---|
PCR | |
Forward | [Btn]GGG TTG GTT GGG TTA GTT TT |
Reverse | ATT CCT AAC TCC TCC ACC CTC TT |
Sequencing | |
Reverse strand | ACC TCA AAC TAA AAC TCT A |
ChIP primers | sequence |
---|---|
-2 | |
Forward | TGC TGA CTG ACG TGG CTA CA |
Reverse | CGC ACC TGG AGT CCA AGA TT |
-1 | |
Forward | GTC CTT GCC ATG AGT CTT GA |
Reverse | CAG GCA GAG CTT ACA GAC AG |
tss | |
Forward | AGC GAA GCC GCT GTG ACG TA |
Reverse | TCT GGG CGC TGG TGG TCT TG |