Skip to main content
. 2018 Jan 9;9(19):14815–14827. doi: 10.18632/oncotarget.24079

Figure 1. The expression of AUF1 and Dicer1 in HCC tissues and cell lines.

Figure 1

(A) Hepatocellular carcinoma (HCC) tissues and the adjacent non-tumor tissues (NC) from HCC patients were subjected to immunohistochemistry staining with anti-AUF1 and anti-Dicer1 antibodies (×400). (B) Total RNA was extracted from HCC tissues and adjacent non-tumor tissues. The relative levels of DICER1 mRNA, AUF1 mRNA, and miR-122 were determined by qRT-PCR compared to GAPDH mRNA. Data are represented as mean ± SD. n = 20; *P < 0.05. (C) HL7702, Huh7, and PLC/PRF/5 cells were cultured in 6-well plate to 80% confluency. Cellular proteins were extracted, and the expression of Dicer1 and AUF1 was determined by Western blotting. AUF1 includes four isoforms (37, 40, 42, and 45 kDa). (D) HHL-5 and Huh7 cells were cultured in 6-well plate to 80% confluency. The level of DICER1 mRNA and AUF1 mRNA was determined by qRT-PCR normalized to GAPDH mRNA. Data are represented as mean ± SD. n = 4, **P < 0.01.