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. 2018 Jan 9;9(19):14815–14827. doi: 10.18632/oncotarget.24079

Figure 2. AUF1 suppresses the expression of Dicer1.

Figure 2

(A, C) PLC/PRF/5 (A) and Huh7 cells (C) were grown in 6-well plate to 70% confluency. Cells were transfected with pEGFP-AUF1 (AUF1) or siRNA of AUF1 (siAUF1) for 48 h. Control cells were transfected with liposome (NC), pEGFP-C1 (C1), and mock siRNA (siMock), respectively. Proteins were extracted and subjected to Western blot analysis. (B, D) The level of DICER1 mRNA was determined by qRT-PCR. Data are represented as mean ± SD. n = 4; **P < 0.01. Experiments were repeated three times and representative results were presented.