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. 2018 Jan 9;9(19):14815–14827. doi: 10.18632/oncotarget.24079

Figure 7. Inhibited expression of AUF1 promotes HCC cell death.

Figure 7

(A) PLC/PRF/5 cells were grown in 6-well plate. The cells were transfected with pmCherry-AUF1 (AUF1) or siRNA of AUF1 (siAUF1) for 24 h in the medium without serum. Apoptosis was determined by flow cytometry. Control cells were transfected with liposome (NC), pmCherry-C1 (C1) or siMock. (B) 80% confluent HHL-5 cells were transfected with siAUF1 or siMock for 24 h in the medium without serum. The expression of Dicer1, AUF1, and cleaved PARP1 was determined by Western blotting (a). The relative expression levels are presented as mean ± SD (b, c, d). n = 4, *P < 0.05, **P < 0.01.