Figure 2. FC-99 inhibited the infiltration and differentiation of monocytes in the liver tissues of septic mice.

All mice were pretreated with FC-99 (10 mg/kgi.p.) 2 h prior to sham operation (sham+FC-99) and/or prior to CLP operation (CLP+FC-99), and/or prior to direct operation (sham, CLP), n = 8. (A) Flow cytometry analysis of the percentage of total monocytes (Lin^-CD11b⁺) and their subtypes (Ly6C^lo/hi) in the peripheral blood of each group 24 h following CLP and/or sham surgery. (B) Flow cytometry analysis of the percentage of lymphocytes (CD45⁺ cells), total myeloid cells (CD45⁺CD11b⁺ cells), monocyte-derived macrophages (CD45⁺CD11b⁺F4/80^lo) and monocytes (CD45⁺CD11b⁺F4/80^-) in the liver tissues of each group at 24 h. (C) Immunofluorescence detection of the apoptotic effects of FC-99 in CD11b⁺ cells in the liver tissues at 24 h post-surgery (magnification 200x). Scale bar, 100 μm. (D) The histogram data of apoptotic CD11b⁺ cells; ^*P < 0.05, ^**P < 0.01, ^***P < 0.005, vs. sham operation group; ^#P < 0.05, ^##P < 0.01, vs. CLP group; ns: no significant difference.