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. 2018 Jan 10;9(19):14959–14976. doi: 10.18632/oncotarget.24127

Figure 3. FC-99 induced THP-1 monocyte apoptosis with PMA stimulation.

Figure 3

(A) Cytotoxicity of FC-99 in THP-1 cells was detected by CCK-8 as described in Material and Methods. FC-99, at several indicated concentrations, was incubated with THP-1 2 h prior to treatment with PMA (2.5 ng/mL) for 48 h and detected by CCK-8. (B) THP-1 cells were divided into two groups: untreated group that comprised control cells treated with PMA (2.5 ng/mL) for 48 h and FC-99-treated group that comprised FC-99 (10μM) treatment 2 h prior to 48 h PMA (2.5 ng/mL) treatment). Flow cytometry with Annexin V/PI results indicated the effects of FC-99 on THP-1-induced apoptosis during differentiation. (C) The levels of the apoptotic marker protein, caspase 3, were examined by western blot analysis. All Results were represented as means ± SEM from four independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001, vs. control group.