Figure 2.

(A) FACS plots showing the method used for hematopoietic progenitors sorting from xenografted bone marrow. Hematopoietic stem cells (HSC) are CD34⁺/CD38^-, common myeloid progenitors (CMP) are CD34⁺/CD38⁺/CD123⁺/CD45RA^-, granulocyte macrophage progenitors (GMP) are CD34⁺/CD38⁺/CD123⁺/CD45RA+, and megakaryocyte–erythroid progenitors (MEP) are CD34⁺/CD38⁺/CD123^-/CD45RA^-. Human cells were previously sorted with human CD34⁺ magnetic beads. (B) Flow chart describing the presence of the different gene mutations in each kind of human progenitors sorted from the bone marrow of the different generations of mice derived from patient 1. (C) Table representing the different variant allele frequency obtained by next generation sequencing on hematopoietic stem cells (HSC) and mesenchymal stromal cells (MSC) from patient 1 xenografted mice. The brown rectangle represents the somatic mutations and the grey one polymorphisms. (D) Scatter plot representing the effect of cytarabine on the percentage of human CD45⁺cells assessed by flow cytometry within the bone marrow retrieved from xenografted mice deriving from patient 1. Two groups, one with mice treated with cytarabine intraperitoneally (10mg/kg) 5 days/7 during two weeks and another group with mock treated mice.