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. 2018 Jan 23;9(19):15036–15046. doi: 10.18632/oncotarget.24542

Figure 4. Inhibition of LPS-induced proinflammatory mediator production by AA in RAW264.7 cells.

Figure 4

(A) Levels of TNF-α, IL-1β and IL-6 in the cell culture medium were determined at 48 h after LPS and AA stimulation. (B) Gene expression levels of TNF-α, IL-1β and IL-6 mRNA were determined by real-time quantitative PCR after AA and LPS stimulation. β-Actin was used as a control. (C) The levels of NO in the cell culture medium and expression of iNOS in cells were determined 48 h after AA and LPS stimulation. Data are shown as mean ± SD (n = 5), and each experiments were duplicated for 3 times. *p < 0.05 versus control, #p < 0.05 versus LPS group.