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. 2018 Jan 23;9(19):15036–15046. doi: 10.18632/oncotarget.24542

Figure 6. AA directly reduced LPS-induced activation of Notch in RAW264.7 cells.

Figure 6

(A) Cells were treated with various concentrations of AA in the absence or presence of LPS for 48 h, followed by analysis of Notch receptors and Dll4 expression by PCR. (B) Protein expression of Notch3 and Hes1 in AA and LPS treatment of Raw264.7 cells. (C) RAW264.7 cells were cultured in the presence of LPS (0.1 μg/mL) in addition to for 48 h. IL-1β and IL-6 expression was determined by qPCR. (D) RAW264.7 cells were cultured in the presence of LPS (0.1 μg/mL) in addition to Dll4 fusion protein (1 μg/mL) for 48 h. IL-1β and IL-6 expression were determined by qPCR. (E) Association of Notch3 and IL-6 gene promoter region was measured in the immunoprecipitate by quantitative PCR relative to that for input DNA. *p < 0.05 versus control, #p < 0.05 versus LPS control. Data are shown as mean ± SD (n = 5), and each experiments were duplicated for 3 times.