Table 1.
NH2-terminal sequences of the acquired proteins by immobilized Trx resin
| Sequences* | Molecular† mass (kDa) [origin] | Predicted proteins | Interaction with Trx | Ref. | Accession number‡ |
|---|---|---|---|---|---|
| AENEEKNTDKWAHLAKDFS | 45.0 | Rubisco activase | Yes | ||
| AENEEKNTDKWAHLAKDFS | 45.7 [Spiol] | 45 kDa | 20 | S45033 | |
| SLQSDHGTVNRVEQLLNLDVTPYT | 44.0 | GS | This study§ | ||
| ALQSDNSTVNRVETLLNLDTKPYS | 41.9 [Arth] | 45 | S69727 | ||
| AENEQKNTDK | 40.5 | Rubisco activase | This study | ||
| AENEEKNTDK | 41.8 [Spiol] | 41 kDa | 20 | S45033 | |
| ALGVAINGFGAIGRNFL | 40.0 | GAPDH | Yes | ||
| KLKVAINGFGRIGRNFL | 36.2 [Spiol] | 14 | X15189 | ||
| ELGDSLNEALAIATTDEGLI | 40.0 | Sedoheptulose 1,7-bisphosphatase (SBPase) | Yes | ||
| ELGDSLEEFLAKATTDKGLI | 34.9 [Spiol] | 37 | L76556 | ||
| SAELPLVGNVAPDFEAGRVF | 25.7 | 2-Cys Prx | Yes | ||
| ADDLPLVGNKAPDFEAEAVF | 21.9 [Spiol] | 25 | X94219 | ||
| STETDVQDVQAEVTSKVFFDVDIGGE | 21.6 | Cyclophilin (PPIase) | This study | ||
| AEEEEVIEPQAKVTNKVYFDVEIGGE | 19.7 [Arth] | 32 | L14845 | ||
| KVKEGTLAPAFSLKXQ | 18.6 | Prx-Q-like protein | Yes | ||
| KVNKGQAAPDFTLKDQ | 17.0 [Arth] | 31 | AB023041 | ||
| PLKAGDIAPKFSLPDQ | 17.4 [Ecoli] | BCP | 51 | AE005477 | |
| MKVXPTQNMVRYETLXYLPP | 14.3 | Rubisco small subunit (rbs2) | This study | X97600 | |
| MKVWPTQNMKRYETLSYLPP | 14.3 [Spiol] |
*
The upper lane is the obtained sequence and the lower sequence is that of the most homologous protein obtained from the databases.
†
The upper value is calculated from SDS/PAGE and the lower one is calculated from the sequence of the indicated protein. The abbreviations used in the brackets were: Spiol, S. oleracea; Arth, A. thaliana; E coli, E. coli.
‡
GenBank accession numbers are indicated.