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Comparison of ATPase activities of RFC, hRad17-RFC, and the 3-subunit complex
| DNA added | RFC | hRad17-RFC | 3S |
|---|---|---|---|
| None | 5.6 | 6.0 | 1.0 |
| Poly dA4000:Oligo dT12–18 | 24.1 | 15.2 | 2.5 |
| φXssDNA | 64.9 | 28.8 | 13.0 |
| φXRFI | 5.7 | 6.3 | 1.1 |
Reactions were carried out as described in Materials and Methods in the presence or absence of 25 μM of the indicated DNA. The values indicate pmol Pi/min per pmol of complex. The 3-subunit complex (3S) is composed of p40, p37, and p36.
