Table 4.
The Time Duration for Cell Cycle Arrest, the Phase at Which the Cell Cycle Was Arrested, Culture Media, and Cell Line Used.
| Author and Year | Culture Media | Cell Line In Vitro, Animal Model In Vivo | Time Duration for Cell Cycle Arrest | Cell Cycle Phase Arrest |
|---|---|---|---|---|
| Giménez et al (2010)8 | RPMI 1640 | In vitro on MHH ES1, MT3 | The IC50 for MHH-ES1 = 17.15 ± 0.82 µM in 30 hours | G2M |
| The IC50 for MT3 = 11.8 ± 1.03 µM in30 hours | ||||
| Lee et al (2011)14 | DMEM | In vitro on RWPE1s | At 24 hours the IC50 with a-tomatine for PC3 (prostrate cancer cells) was 1.67 ± 0.3 µM | G2M |
| Kim et al (2012)11 | DMEM | In vitro on SKOV-3 | The SKOV-3 cells incubated with PAC1 at 75 µg/mL in 24 hours, reduced its proliferation by 64.1% | G2M |
| Meng et al (2011)19 | DMEM | In vitro on MDA MB 231 | Treating MDA MB-231 cells with 0.1, 0.2, 0.4 mg/mL ECMS for 48 hours increased the proportion for apoptotic cells from 3.1% to 33% | G2M |
| Torres et al (2012)28 | DMEM | In vitro on FG/COL357 and CD18/HPAF and in vivo on athymic nude mice | At 48 hours the IC50 of graviola on FG/COLO357 was 200 µg/mL and CD18/HPAF was 73 µg/mL | G2M |
| Elias et al (2013)20 | DMEM | In vitro on MCF7, OSCC3 | The MCF7 cells seeded at 1.5 × 104 cells/well density on 24 well plates were treated with 500 mg/mL of P tortua extract at 24 and 48 hours caused death of 60% to 67% of cells | G1 |
| Fan et al (2014)7 | DMEM | In vitro on HepG2 | At 24 hours 30 µg/mL curcumin suppressed HepG2 cell growth by 93% | G2M |
| Edderkaoui et al (2013)6 | DMEM | In vitro on PaCa-2, HPAF-II cell lines and in vivo on athymic nude mice | At 72 hours, ellagic acid and embellin caused decreased cell proliferation at 0.5 µM in MIA PaCa-2, HPAF-II at 1 µM | G2M |
| Zhu et al (2013)33 | DMEM | In vitro on SW629 | At 48 hours, 150 mg/mL PEEP caused increase in apoptosis from 4.8% to 45% | G2M |
| Pan et al (2013)22 | FBS | In vitro on HUVEC, SW-620, HCT-116 cell lines and nude mice in vivo | Aloin at 200-240 µmol/L showed an IC50, the apoptotic activity increased by 33% in 72 hours | G1 |
| Yen et al (2014)30 | DMEM | In vitro on Ca 922 | After 24 hours of treatment, the cell viability drastically dropped from 100 ± 6.3 to 3.7 ± 1.3 at concentrations ranging from 0.05 to 1 mg/mL | G1 |
| Xiao et al (2014)29 | RPMI1640 | In vitro on MDA-MB 231 and in vivo on nude mice | The cancer cells show growth inhibition of 34.2% with 5 mg/L at 96 hours, the IC50 of DADS was 15 mg/L at 96 hours | G2M |
| Arumugam et al (2014)2 | In vivo study on Sprague-Dawley rats | At 48-72 hours apoptotic changes increased significantly than that at 24 hours in a dose-dependent manner | G2M | |
| Ling et al (2014)15 | RPMI1640 | In vitro on MGC803 | 30 mg/mL DADS enhanced phospho-Chk1 protein levels in a time dependent pattern in 12 hours | G2M |
| Sodde et al (2015)25 | DMEM | In vitro on MCF7 | In 48 hours M parasiticus showed IC50 59.33 ± 3.3 μg/mL | G2M |
| Samal et al (2015)23 | DMEM | In vitro on H357 | In 48 hours, the cell growth was declined by 1 μM; the IC50 was 2.6 μM | G2M |
| Akimoto et al (2015)1 | DMEM, FBS | In vitro on Panc1 | When Panc1 cells were treated with ginger extract 200 μg/mL for 20 hours causes cell cycle arrest | G0-G1 |
| Bassa et al (2016)4 | DMEM | In vitro on MCF7 | Following a 24 hour treatment, the proliferation of cancer cells was reduced by 50% | G2M |
| Kala et al (2015)10 | RPMI1640 | In vitro on MDA MB 157, HCC1806 | In 24 hours, there was an ER transcriptional activation, greater duration suppressed the activity | G2M, S |
| Bishayee et al (2016)5 | DMEM | In vitro on HCC1806, MDA MB 157 cancer cell Sprague-Dawley rat | In 72 hours, HCC1806 cancer cells were arrested at G2M phase, MDA MB 157 cancer cells with 15 μM combination treatment | G2M |
| At 5 g/kg tumor incidence was reduced by 54% |
Abbreviations: DMEM, Dulbecco modified Eagles medium; FBS, fetal bovine serum; PEEP, polyphenol extract of Phyllanthus emblica; RPMI1640, Roswell Park Memorial Institute Medium.