Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (human) | SPIN1 | National Center for Biotechnology Information (https://www.ncbi.nlm.nih.gov/gene/10927) | Gene ID: 10927; Accession number: NM_006717; UniPro ID: Q9Y657 | |
| Gene (human) | RPL5/uL18 | National Center for Biotechnology Information https://www.ncbi.nlm.nih.gov/gene/6125 | gene ID: 6125; Accession number: NM_000969; UniPro ID: P46777 | |
| Gene (human) | RPL11/uL5 | National Center for Biotechnology Information https://www.ncbi.nlm.nih.gov/gene/6135 | gene ID: 6135; Acctssion number: NM_000975; UniPro ID: P62913 | |
| Gene (human) | RPL23/uL14 | National Center for Biotechnology Information https://www.ncbi.nlm.nih.gov/gene/9349 | gene ID: 9349; Accession number: NM_000978; UniPro ID: P62829 | |
| Gene (human) | TP53 | National Center for Biotechnology Information https://www.ncbi.nlm.nih.gov/gene/7157 | gene ID: 7157; Accession number: NM_000546; UniPro ID: P04637 | |
| Gene (human) | p21/CDKN1A | National Center for Biotechnology Information https://www.ncbi.nlm.nih.gov/gene/1026 | gene ID: 1026; Accession number: NM_000389; UniPro ID: Q42580 | |
| Gene (human) | PUMA/BBC3 | National Center for Biotechnology Information https://www.ncbi.nlm.nih.gov/gene/27113 | gene ID: 27113; Accession number: NM_001127240; UniPro ID: Q9BXH1 | |
| Gene (human) | MDM2 | National Center for Biotechnology Information https://www.ncbi.nlm.nih.gov/gene/4193 | gene ID: 4193; Accession number: NM_001145337; UniPro ID: Q00987 | |
| Strain, strain background (mouse) | NOD-SCID | Jackson Laboratories https://www.jax.org/strain/001303 | Stock No: 001303 | |
| Cell line (human) | 293 | ATCC https://www.atcc.org/Products/All/CRL-1573.aspx | Catalog number: ATCC CRL-1573; RRID:CVCL_0045 | |
| Cell line (human) | H1299 | ATCC https://www.atcc.org/Products/All/CRL-5803.aspx | Catalog number: ATCC CRL-5803; RRID: CVCL_0060 | |
| Cell line (human) | U2OS | ATCC https://www.atcc.org/Products/All/HTB-96.aspx | Catalog number: ATCC HTB-96; RRID:RRID:CVCL_0042 | |
| Cell line (human) | H460 | ATCC https://www.atcc.org/Products/All/HTB-177.aspx | Catalog number: ATCC HTB-177; RRID:CVCL_0459 | |
| Cell line (human) | HCT116 p53+/+ | from Dr. Bert Vogelstein at the John Hopkins Medical institutes | ||
| Cell line (human) | HCT116 p53-/- | from Dr. Bert Vogelstein at the John Hopkins Medical institutes | ||
| Cell line (human) | MEF (Mdm2-/-; p53-/-) | from Dr. Guillermina Lozano from MD Anderson Cancer Center, the University of Texas. | ||
| Antibody | Mouse anti-human Flag monoclonal antibody | Sigma-Aldrich | Catalog number: F1804; RRID: AB_262044 | Applications: WB; Immunofluoresce |
| Antibody | Mouse anti-human Myc monoclonal antibody | Santa Cruz Technology | Catalogue number: sc-40 | Applications: WB; Immunofluoresce |
| Antibody | Mouse anti-human GFP monoclonal antibody | Santa Cruz Technology | Catalogue number: sc-9996; RRID: AB_627695 | Applications: WB; Immunofluoresce |
| Antibody | Mouse anti-human GST monoclonal | ProteinTech | Catalogue number: HRP-66001; RRID: AB_10951482 | Applications: WB |
| Antibody | Rabbit anti-bacterial His polyclonal antibody | ProteinTech | Catalogue number:10560–1-lg; RRID: AB_1607770 | Applications: WB |
| Antibody | Rabbit anti-human SPIN1 polyclonal antibody | ProteinTech | Catalogue number:12105–1-AP; RRID: AB_2196111 | Applications: WB |
| Antibody | Mouse anti-human p53 monoclonal antibody | Santa Cruz Technology | Catalogue number: sc-126; RRID: AB_628082 | Applications: WB |
| Antibody | Mouse anti-human p21 monoclonal antibody | Neomarkers, Fremont, | Catalogue number: MS-891-P0; RRID:AB_143907 | Applications: WB |
| Antibody | Rabbit anti-human PUMA polyclonal antibody | ProteinTech | Catalogue number:55120–1-AP; RRID:AB_10859944 | Applications: WB |
| Antibody | Mouse anti-human β-actin monoclonal antibody | Santa Cruz Technology | Catalogue number: sc-47778; RRID:AB_2714189 | Applications: WB |
| Antibody | Rabbit anti-human GAPDH polyclonal antibody | Proteintech | Catalogue number:10494–1-AP; RRID:AB_2263076 | Applications: WB |
| Chemical compound, drug | Cycloheximide | Sigma-Aldrich | Catalogue number: 66-81-9 | |
| Chemical compound, drug | MG-132 | Sigma-Aldrich | Catalogue number:474787 | |
| Chemical compound, drug | 5-FU | Sigma-Aldrich | Catalogue number:51218 | |
| Chemical compound, drug | Actinomycin D (Act D) | Sigma-Aldrich | Catalogue number: 50-76-0 |