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. Author manuscript; available in PMC: 2018 Mar 27.
Published in final edited form as: Cancer. 2010 Nov 8;117(8):1649–1660. doi: 10.1002/cncr.25666

Figure 4. Apoptosis assay with stably (A-498) or transient (Caki-1) transfected empty and DKK4 renal cancer cells and protein expression of apoptosis markers.

Figure 4

A. Flow cytometry analysis of apoptosis in empty vector and stable DKK4 clone 1 and clone 2 cells and transient DKK4 transfectants of Caki-1 cells. Annexin V-FITC and 7-AAD were measured by flow cytometry. Representative results are also shown (B). Data are the mean ± S.D. of four independent experiments. C. Protein expression of pro-caspase-3 and cleaved caspase3 in empty and DKK4 stable transfectants (A-498) and DKK4 transient transfectants (Caki-1).