Fig. 3.

Expression of inflammatory mediators and neutrophil recruitment in the liver 4 h after intracerebral injection of 1 ng, 10 ng or 100 ng IL-1β at P14. IL-6, TNF, ICAM-1, CXCL-2 and CXCL-5 mRNA expression in the liver significantly increases following intracerebral injection of 100 ng of IL-1β, but not after the 1 ng dose (A). Data are represented as relative fold change from expression levels of naïve mice P14. Neutrophil immunohistochemistry, counterstained with cresyl violet, in the liver shows significant increases in the number of neutrophils per mm² in tissue following the intracerebral injection of 10 ng and 100 ng IL-1β at P14. Scale bar = 50 µm. ^*p < .05, ^**p < .01 and ^***p < .001. Naïve, 1 ng, 10 ng and 100 ng: n = 4, and saline: n = 8 for RT-qPCR; naïve: n = 7, saline: n = 6, 1 ng, 10 ng and 100 ng: n = 3 for immunohistochemistry analysis. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)