Supplementary Fig. A3.

The expression of inflammatory mediators in the liver in response to a low or high dose of IL-1β in the brain during postnatal period. IL-6, TNF, ICAM-1, CXCL-2 and CXCL-5 mRNA expression in the liver of naïve mice and mice that received intracerebral injection of saline or 1ng IL-1β or 100 ng IL-1β at different postnatal ages (P7, P14, P21 and P56). Data are represented as relative fold change from expression levels of P56 mice. At all ages, there was a significant increase in liver gene expression of inflammatory molecules 4 h after the 100 ng intracerebral injection. The 1ng dose only caused an increase in gene expression at P56 for TNF. ^∗p < 0.05, ^∗∗p < 0.01 and ^∗∗∗p < 0.001. P7: n = 4 for saline, n = 5 for 1ng and 100 ng IL-1β; P14: n = 4 for saline, n = 3 for 1ng IL-1β, n = 5 for 100 ng IL-1β, P21: n = 4 for saline, 1ng IL-1β and 100 ng IL-1β; P56: n = 4 for saline and 1ng IL-1β, n = 5 for 100 ng IL-1β.