Supplementary Fig. A5.

mRNA expression of inflammatory mediators and neutrophil population in the liver during postnatal development. (A) IL-6, TNF, ICAM-1, CXCL-2 and CXCL-5 mRNA expression in the liver of naïve mice at different postnatal ages (P7, P14, P21 and P56). Data are represented as relative fold change from expression levels of P56 mice. For most genes, the highest expression was measured at P7 and was reduced during further postnatal development. No change in CXCL-2 was found in the liver over the developmental period studied, while the expression of CXCL-5 remained high until P21 and was significantly reduced at P56. The number of neutrophils per mm² of liver also decreased during postnatal development (B, C). Scale bar = 50 µm. ^∗p < 0.05, ^∗∗p < 0.01 and ^∗∗∗p < 0.001. P7: n = 4, P14: n = 4, P21 n = 4 and P56: n = 3.