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. Author manuscript; available in PMC: 2018 Mar 28.
Published in final edited form as: Mol Pharm. 2017 Sep 13;14(10):3499–3511. doi: 10.1021/acs.molpharmaceut.7b00553

Figure 5.

Figure 5

Time-dependent modification of Hb A in normal blood incubated with test compounds. Normal blood from healthy donors was incubated with 2 mM test molecules for 24 h, during which aliquot samples were collected, reduced, and stored until batch analysis by cation exchange HPLC. Levels of modified Hb peaked between 2 and 4 h for all compounds followed by a decline, suggesting that the compounds are subject to metabolism by RBC enzymes. For the esters and alkyl ethers, there was ~92% decline in adduct formation at 24 h, similar to 5-HMF (a,b), while the aryl ethers showed only ~70% decline (c).