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. Author manuscript; available in PMC: 2018 May 21.
Published in final edited form as: Leukemia. 2017 Nov 21;32(4):996–1002. doi: 10.1038/leu.2017.334

Fig. 6. PRMT5 inhibition by EPZ015666 induces autophagic proteolysis of IKKβ.

Fig. 6

a–b, H929 were treated with EPZ015666 for 72h in the presence of 0.5 mmol/l of 3-MA. Cell lysates were immunoprecipitated with: (a) IKKβ antibody, and then immunoblotted for Ubiquitin and TRIM21 or (b) TRIM21 antibody, and then immunoblotted for phospho-Beclin1, IKKβ, and TRIM21. c, H929 were treated with EPZ015666. After treatment, samples dual stained with Cyto-ID Green dye and IKKβ antibody were analyzed by fluorescence microscopy. Arrows indicate colocalization of IKKβ in the autophagosome. d, MTT assay of H929 treated with EPZ015666 in presence or absence of 0.5 mmol/l of 3-MA. e, Proposed model of PRMT5/TRIM21/IKKB axis in MM. Data are representative of at least two independent experiments. * = p<0.05