Table 2.
Summary of results obtained by amoebal co culture of blood and organs of rats and mice inoculated with Marseillevirus.
| Coculture | Day 0 | Day 1 | Day 2 | Day 3 | Day 7 | Day 14 | Day 21 | Day 30 | Day 43 | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| IP route | Rats | Blood | Positive (n = 1/2) | Positive (n = 1/4) | ND | Negative (n = 0/2) | Negative (n = 0/4) | Negative (n = 0/3) | ND | ND | Negative (n = 0/1) |
| Spleen | ND | Positive (n = 2/2) | ND | Positive (n = 1/2) | Positive (n = 3/4) | Positive (n = 3/3) | ND | ND | Negative (n = 0/1) | ||
| Liver | ND | Positive (n = 2/2) | ND | Negative (n = 0/2) | Positive (n = 1/4) | Negative (n = 0/3) | ND | ND | Negative (n = 0/1) | ||
| Mice | Blood | Positive (n = 3/4) | Positive (n = 1/4) | Negative (n = 0/3) | ND | Negative (n = 0/3) | ND | Negative (n = 0/1) | ND | ND | |
| Spleen | Positive (n = 4/4) | Positive (n = 4/4) | Positive (n = 2/3) | ND | Positive (n = 2/3) | ND | Negative (n = 0/1) | ND | ND | ||
| Liver | ND | ND | ND | ND | ND | ND | ND | ND | ND | ||
| IV route | Rats | Blood | ND | Positive (n = 4/4) | Positive (n = 2/2) | Negative (n = 0/3) | Negative (n = 0/3) | Negative (n = 0/3) | Negative (n = 0/3) | Negative (n = 0/3) | Negative (n = 0/2) |
| Spleen | ND | Positive (n = 4/4) | ND | Positive (n = 2/3) | Positive (n = 2/3) | Positive (n = 3/3) | Positive (n = 2/2) | Negative (n = 0/3) | Negative (n = 0/2) | ||
| Liver | ND | Positive (n = 4/4) | ND | Positive (n = 3/3) | Positive (n = 2/3) | Positive (n = 2/3) | Negative (n = 0/2) | Negative (n = 0/3) | Negative (n = 0/2) | ||
| Aerosol route | Mice | Blood | Negative (n = 0/4) | Negative (n = 0/5) | ND | ND | Negative (n = 0/18) | Negative (n = 0/15) | Negative (n = 0/14) | Negative (n = 0/5) | ND |
| Spleen | Negative (n = 0/4) | Negative (n = 0/5) | ND | ND | Negative (n = 0/18) | Negative (n = 0/15) | Negative (n = 0/14) | Negative (n = 0/5) | ND | ||
| Liver | Negative (n = 0/4) | Negative (n = 0/5) | ND | ND | Negative (n = 0/18) | Negative (n = 0/15) | Negative (n = 0/14) | Negative (n = 0/5) | ND | ||
| Lung | Positive (n = 4/4) | Positive (n = 15/15) | ND | ND | Positive (n = 16/18) | Positive (n = 8/15) | Positive (n = 1/14) | Positive (n = 1/4) | ND | ||
| NALT | Positive (n = 2/2) | Positive (n = 11/11) | ND | ND | Positive (n = 18/18) | Positive (n = 14/15) | Positive (n = 11/14) | Positive (n = 3/3) | ND |
For some times of evaluation, the number of samples tested by coculture is different from those appearing in the table showing PCR results because of either insufficient quantity or ininterpretable results of PCR (negativity for internal control DNA and Marseillevirus DNA). In each case, the second line is the number of positive samples / number of tested samples. ND = Not done.