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. 2018 Mar 21;9:507. doi: 10.3389/fimmu.2018.00507

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Copyright © 2018 Casucci, Falcone, Camisa, Norelli, Porcellini, Stornaiuolo, Ciceri, Traversari, Bordignon, Bonini and Bondanza.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lack of activation of nerve-growth-factor receptor (NGFR)-enriched CD44v6 chimeric antigen receptor (CAR)-T cells in response to NGF. NGFR isoform-enriched CD44v6 CAR-T cells were labeled with CFSE and exposed to increasing concentrations of soluble NGF. CD44v6+ MM.1S and CD44v6− BV-173 cells were used as positive and negative stimuli, respectively. After 6 days, CAR-T cell proliferation was analyzed by FACS and expressed as percentages (inserts) of CFSE-diluting cells. Upper panel: FACS plots of CAR-T cells from a representative donor. Lower panel: percentages of CFSE-diluting cells from n = 3 donors (means ± SEM). Results from a one-way ANOVA test are shown when statistically significant (*P ≤ 0.05; **P ≤ 0.01; ****P ≤ 0.0001).