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. 2018 Mar 21;9:372. doi: 10.3389/fpls.2018.00372

FIGURE 3.

FIGURE 3

EAR and RLFGI motifs are required for the interaction of ARF2 with TPL/TPRs in the yeast cell. (A) Schematic representations of ARF2 full domains (Full), domain deletions (DBD, MD, PB1), and motif-mutated middle domains (MDma, MDmb, and MDmab). (B) Yeast two-hybrid assay between TPL and ARF2 domains. TPL is fused with GAL4 DNA binding domain (DB), and full length and domain-deleted ARF2 are fused with GAL4 activation domain (AD). (C) Yeast two-hybrid assay between TPL and mutated ARF2-MD series. TPL is fused with GAL4-BD, and ARF2-MD series are fused with GAL4-AD. (D) Yeast two-hybrid assay between ARF2-MD (MD) and TPL/TPRs. MD is fused with GAL4-BD, and TPL/TPRs are fused with GAL4-AD (NC, negative control; PC, positive control as described in section “Materials and Methods”). Empty indicates no TPL in the BD-fusion (B,C), and no ARF2 domain (B) or no MD (C) or no TPL/TPR (D) in the AD fusion.