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. 2018 Mar 21;9:372. doi: 10.3389/fpls.2018.00372

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Copyright © 2018 Choi, Seo and Cho.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The determination of seed size by ARF2 requires its TPL/TPR-binding motifs. (A) Representative seed images of wild type (Cont), arf2 mutants, and the arf2 mutants complemented with wild-type (ARF2) or mutated (ma, mb, and mab) ARF2 under ProARF2. Bar is 500 μm. (B,C) Quantitative seed size analyses of the lines shown in A. Seed size was quantified as the area of the two-dimensional seed image. Data represent means ± SE (n = 24–244 seeds from 3 to 7 independent lines for transformants). Statistically significant differences are denoted with different letters (one-way ANOVA with Tukey’s unequal N-HSD post hoc test, P < 0.05).