Table 1.
Identified glycoproteins carrying oligomannose N-glycans.
| Peptide <ProteinMetrics confidential> | Glycans NHFAGNa | Observed m/z | z | Observed (M + H) | Calc. mass (M + H) | Mass error (ppm) | Cleavage | Score |
|---|---|---|---|---|---|---|---|---|
| Complement C3 | P01024 | |||||||
| K.TVLTPATN[+0.984]HM[+15.995]GN[+1,378.476]VTFTIPANR.E | HexNAc(2)Hex(6) | 1,217.553 | 3 | 3,650.644 | 3,650.615 | 8.1 | Specific | 124.0 |
| K.TVLTPATN[+0.984]HMGN[+1,378.476]VTFTIPANR.E | HexNAc(2)Hex(6) | 1,212.217 | 3 | 3,634.635 | 3,634.620 | 4.2 | Specific | 379.1 |
| K.TVLTPATN[+0.984]HMGN[+1,540.529]VTFTIPANR.E | HexNAc(2)Hex(7) | 1,266.237 | 3 | 3,796.695 | 3,796.673 | 5.8 | Specific | 384.1 |
| K.TVLTPATNHM[+15.995]GN[+1,216.423]VTFTIPANR.E | HexNAc(2)Hex(5) | 1,163.202 | 3 | 3,487.590 | 3,487.578 | 3.6 | Specific | 141.1 |
| K.TVLTPATNHM[+15.995]GN[+1,378.476]VTFTIPANR.E | HexNAc(2)Hex(6) | 1,217.217 | 3 | 3,649.636 | 3,649.631 | 1.5 | Specific | 362.2 |
| K.TVLTPATNHMGN[+1,378.476]VTFTIPANR.E | HexNAc(2)Hex(6) | 909.165 | 4 | 3,633.637 | 3,633.636 | 0.3 | Specific | 555.7 |
| K.TVLTPATNHMGN[+1,540.529]VTFTIPANR.E | HexNAc(2)Hex(7) | 1,265.903 | 3 | 3,795.696 | 3,795.689 | 1.9 | Specific | 497.1 |
| K.TVLTPATNHMGN[+1,864.634]VTFTIPANR.E | HexNAc(2)Hex(9) | 1,374.273 | 3 | 4,120.805 | 4,119.794 | 1.8 | Specific | 107.6 |
| K.VVPEGIRMN[+1,702.581]K.T | HexNAc(2)Hex(8) | 949.077 | 3 | 2,845.216 | 2,845.216 | 0.0 | Specific | 142.1 |
| R.MN[+1,702.581]KTVAVR.T | HexNAc(2)Hex(8) | 874.373 | 3 | 2,621.105 | 2,621.100 | 1.7 | Specific | 328.3 |
| R.MN[+1,864.634]KTVAVR.T | HexNAc(2)Hex(9) | 928.391 | 3 | 2,783.159 | 2,783.153 | 2.2 | Specific | 299.3 |
| Laminin subunit gamma-1 | P11047 | |||||||
| R.RVN[+0.984]DN[+1,540.529]KTAAEEALR.K | HexNAc(2)Hex(7) | 782.841 | 4 | 3,128.343 | 3,128.337 | 2.0 | Specific | 200.0 |
| K.TAN[+1,540.529]DTSTEAYNLLLR.T | HexNAc(2)Hex(7) | 1,074.797 | 3 | 3,222.377 | 3,222.368 | 3.0 | Specific | 249.2 |
| K.TAN[+1,216.423]DTSTEAYNLLLR.T | HexNAc(2)Hex(5) | 966.761 | 3 | 2,898.269 | 2,898.262 | 2.4 | Specific | 743.2 |
| K.LLNN[+1,216.423]LTSIK.I | HexNAc(2)Hex(5) | 1,116.524 | 2 | 2,232.040 | 2,232.038 | 1.3 | Specific | 98.8 |
| K.LLNN[+1,378.476]LTSIK.I | HexNAc(2)Hex(6) | 1,197.552 | 2 | 2,394.096 | 2,394.090 | 2.3 | Specific | 84.4 |
| K.LLNN[+1,216.423]LTSIK.I | HexNAc(2)Hex(5) | 1,116.524 | 2 | 2,232.041 | 2,232.038 | 1.4 | Specific | 110.9 |
| Serpin H1 | P50454 | |||||||
| R.N[+1,378.476]VTWK.L | HexNAc(2)Hex(6) | 1,013.420 | 2 | 2,025.832 | 2,025.827 | 2.8 | Specific | 267.7 |
| R.N[+1,444.534]VTWK.L | HexNAc(4)Hex(3)Fuc(1) | 1,046.447 | 2 | 2,091.887 | 2,091.885 | 1.0 | Specific | 163.0 |
| R.N[+1,540.529]VTWK.L | HexNAc(2)Hex(7) | 1,094.447 | 2 | 2,187.886 | 2,187.880 | 2.8 | Specific | 244.1 |
| R.N[+1,702.581]VTWK.L | HexNAc(2)Hex(8) | 1,175.471 | 2 | 2,349.936 | 2,349.933 | 1.3 | Specific | 272.5 |
| R.N[+1,864.634]VTWK.L | HexNAc(2)Hex(9) | 1,256.498 | 2 | 2,511.989 | 2,511.985 | 1.6 | Specific | 216.2 |
| R.SLSN[+1,241.454]STAR.N | HexNAc(3)Hex(3)Fuc(1) | 1,038.946 | 2 | 2,076.886 | 2,076.881 | 2.0 | Specific | 125.8 |
| R.SLSN[+1,378.476]STAR.N | HexNAc(2)Hex(6) | 1,107.458 | 2 | 2,213.909 | 2,213.903 | 3.0 | Specific | 228.1 |
| Peptidyl-prolyl cis–trans isomerase | Q96AY3 | |||||||
| R.YHYN[+1,378.476]C[+57.021]SLLDGTQLFTSHDYGAPQEATLGANK.V | HexNAc(2)Hex(6) | 1,213.525 | 4 | 4,851.079 | 4,850.067 | 1.8 | Specific | 324.9 |
| R.YHYN[+1,378.476]GTFEDGKK.F | HexNAc(2)Hex(6) | 710.041 | 4 | 2,837.141 | 2,837.141 | 0.1 | Specific | 346.6 |
| R.YHYN[+1,702.581]GSLMDGTLFDSSYSR.N | HexNAc(2)Hex(8) | 1,305.853 | 3 | 3,915.543 | 3,915.542 | 0.3 | Specific | 365.9 |
| R.YHYN[+1,702.581]GTLLDGTSFDTSYSK.G | HexNAc(2)Hex(8) | 1,291.193 | 3 | 3,871.566 | 3,871.558 | 1.8 | Specific | 396.5 |
| R.YHYN[+1,864.634]GSLMDGTLFDSSYSR.N | HexNAc(2)Hex(9) | 1,359.874 | 3 | 4,077.607 | 4,077.595 | 3.1 | Specific | 355.7 |
| R.YHYN[+1,864.634]GTLLDGTSFDTSYSK.G | HexNAc(2)Hex(9) | 1,345.549 | 3 | 4,034.632 | 4,033.611 | 4.2 | Specific | 291.9 |
| Endoplasmin | P14625 | |||||||
| K.HN[+0.984]N[+1,216.423]DTQHIWESDSNEFSVIADPR.G | HexNAc(2)Hex(5) | 982.919 | 4 | 3,928.654 | 3,928.615 | 9.8 | Specific | 78.0 |
| K.HN[+0.984]N[+1,378.476]DTQHIWESDSNEFSVIAD PR.G | HexNAc(2)Hex(6) | 1,023.678 | 4 | 4,091.690 | 4,090.668 | 4.6 | Specific | 187.1 |
| K.HNN[+1,378.476]DTQHIWESDSNEFSVIADPR.G | HexNAc(2)Hex(6) | 1,023.178 | 4 | 4,089.691 | 4,089.684 | 1.9 | Specific | 340.4 |
| K.HNN[+1,702.581]DTQHIWESDSNEFSVIADPR.G | HexNAc(2)Hex(8) | 1,104.458 | 4 | 4,414.810 | 4,413.789 | 4.0 | Specific | 353.4 |
| Microfibril-associated glycoprotein-4 | P55083 | |||||||
| R.FN[+1,216.423]GSVSFFR.G | HexNAc(2)Hex(5) | 1,138.977 | 2 | 2,276.946 | 2,276.944 | 0.9 | Specific | 243.7 |
| R.FN[+1,378.476]GSVSFFR.G | HexNAc(2)Hex(6) | 1,220.004 | 2 | 2,439.000 | 2,438.997 | 1.4 | Specific | 308.4 |
| R.FN[+1,378.476]GSVSFFR.G | HexNAc(2)Hex(6) | 1,220.005 | 2 | 2,439.003 | 2,438.997 | 2.5 | Specific | 178.0 |
| R.FN[+1,540.529]GSVSFFR.G | HexNAc(2)Hex(7) | 1,301.031 | 2 | 2,601.055 | 2,601.050 | 2.1 | Specific | 100.0 |
| R.VDLEDFEN[+1,768.640]NTAYAK.Y | HexNAc(4)Hex(5)Fuc(1) | 1,133.136 | 3 | 3,397.392 | 3,397.383 | 2.5 | Specific | 53.2 |
| R.VDLEDFEN[+1,768.640]NTAYAK.Y | HexNAc(4)Hex(5)Fuc(1) | 1,133.133 | 3 | 3,397.384 | 3,397.383 | 0.0 | Specific | 132.2 |
| R.VDLEDFEN[+1,768.640]NTAYAK.Y | HexNAc(4)Hex(5)Fuc(1) | 1,133.133 | 3 | 3,397.385 | 3,397.383 | 0.4 | Specific | 327.8 |
| R.VDLEDFEN[+2,059.735]NTAYAK.Y | HexNAc(4)Hex(5)Fuc(1)NeuAc(1) | 1,230.166 | 3 | 3,688.483 | 3,688.479 | 1.2 | Specific | 159.8 |