Table 2.
Identified glycoproteins carrying sialylated and core-fucosylated N-glycans.
| Peptide <ProteinMetrics confidential> | Glycans NHFAGNa | Observed m/z | z | Observed (M + H) | Calc. mass (M + H) | Mass error (ppm) | Cleavage | Score |
|---|---|---|---|---|---|---|---|---|
| Decorin | P07585 | |||||||
| R.IADTN[+1,913.677]ITSIPQGLPPSLTELHLDGNK.I | HexNAc(4)Hex(5)NeuAc(1) | 1,165.290 | 4 | 4,658.137 | 4,658.124 | 2.9 | Specific | 126.1 |
| K.LGLSFNSISAVDN[+2,350.830]GSLANTPHLR.E | HexNAc(4)Hex(5)Fuc(1)NeuAc(2) | 1,184.525 | 4 | 4,735.077 | 4,734.067 | 1.4 | Specific | 113.9 |
| K.LGLSFNSISAVDN[+2,059.735]GSLANTPHLR.E | HexNAc(4)Hex(5)Fuc(1)NeuAc(1) | 1,111.751 | 4 | 4,443.981 | 4,442.971 | 1.3 | Specific | 396.3 |
| K.LGLSFNSISAVDN[+1,768.640]GSLANTPHLR.E | HexNAc(4)Hex(5)Fuc(1) | 1,038.726 | 4 | 4,151.884 | 4,151.876 | 1.8 | Specific | 165.8 |
| K.LGLSFN[+0.984]SISAVDN[+2,059.735]GSLANTPHL R.E | HexNAc(4)Hex(5)Fuc(1)NeuAc(1) | 1,111.744 | 4 | 4,443.955 | 4,443.955 | 0.0 | Specific | 244.7 |
| R.IADTN[+1,419.502]ITSIPQGLPPSLTELHLDGNK.I | HexNAc(3)Hex(5) | 1,041.743 | 4 | 4,163.950 | 4,163.949 | 0.4 | Specific | 266.0 |
| R.IADTN[+1,257.449]ITSIPQGLPPSLTELHLDGNK.I | HexNAc(3)Hex(4) | 1,001.231 | 4 | 4,001.902 | 4,001.896 | 1.5 | Specific | 296.2 |
| R.IADTN[+1,622.582]ITSIPQGLPPSLTELHLDGNK.I | HexNAc(4)Hex(5) | 1,092.514 | 4 | 4,367.032 | 4,367.028 | 1.0 | Specific | 396.0 |
| K.LGLSFNSISAVDN[+2,059.735]GSLANTPHLR.E | HexNAc(4)Hex(5)Fuc(1)NeuAc(1) | 1,111.501 | 4 | 4,442.983 | 4,442.971 | 2.6 | Specific | 292.4 |
| K.LGLSFNSISAVDN[+1,768.640]GSLANTPHLR.E | HexNAc(4)Hex(5)Fuc(1) | 1,038.726 | 4 | 4,151.884 | 4,151.876 | 1.8 | Specific | 493.3 |
| K.LGLSFN[+0.984]SISAVDN[+1,768.640]GSLANTPHL R.E | HexNAc(4)Hex(5)Fuc(1) | 1,038.972 | 4 | 4,152.866 | 4,152.860 | 1.4 | Specific | 517.4 |
| Collagen alpha-2(VI) | P12110 | |||||||
| R.GTFTDC[+57.021]ALAN[+1,622.582]MTEQIR.Q | HexNAc(4)Hex(5) | 1,150.813 | 3 | 3,450.426 | 3,450.418 | 2.2 | Specific | 191.3 |
| R.N[+1,768.640]FTAADWGQSR.D | HexNAc(4)Hex(5)Fuc(1) | 1,007.742 | 3 | 3,021.212 | 3,021.210 | 0.8 | Specific | 316.8 |
| R.N[+1,768.640]M[+15.995]TLFSDLVAEK.F | HexNAc(4)Hex(5)Fuc(1) | 1,051.448 | 3 | 3,152.328 | 3,152.322 | 1.9 | Specific | 102.2 |
| R.N[+1,768.640]MTLFSDLVAEK.F | HexNAc(4)Hex(5)Fuc(1) | 1,046.116 | 3 | 3,136.334 | 3,136.327 | 2.4 | Specific | 225.4 |
| R.N[+2,059.735]FTAADWGQSR.D | HexNAc(4)Hex(5)Fuc(1)NeuAc(1) | 1,104.773 | 3 | 3,312.306 | 3,312.305 | 0.1 | Specific | 157.1 |
| R.N[+2,059.735]M[+15.995]TLFSDLVAEK.F | HexNAc(4)Hex(5)Fuc(1)NeuAc(1) | 114 8.478 | 3 | 3,443.419 | 3,443.417 | 0.4 | Specific | 122.5 |
| R.N[+2,059.735]MTLFSDLVAEK.F | HexNAc(4)Hex(5)Fuc(1)NeuAc(1) | 114 3.148 | 3 | 3,427.430 | 3,427.422 | 2.3 | Specific | 154.5 |
| R.N[+2,350.830]FTAADWGQSR.D | HexNAc(4)Hex(5)Fuc(1)NeuAc(2) | 1,201.806 | 3 | 3,603.404 | 3,603.401 | 0.9 | Specific | 109.0 |
| R.N[+2,350.830]M[+15.995]TLFSDLVAEK.F | HexNAc(4)Hex(5)Fuc(1)NeuAc(2) | 1,245.512 | 3 | 3,734.521 | 3,734.513 | 2.1 | Specific | 128.6 |
| R.RN[+892.317]FTAADWGQSR.D | HexNAc(2)Hex(3) | 767.669 | 3 | 2,300.993 | 2,300.989 | 1.7 | Specific | 30.0 |
| Isoform 2 of collagen alpha-3(VI) | P12111-2 | |||||||
| R.QLINALQIN[+1,768.640]NTAVGHALVLPAGR.D | HexNAc(4)Hex(5)Fuc(1) | 1,038.756 | 4 | 4,152.002 | 4,151.996 | 1.3 | Specific | 590.4 |
| R.QLINALQIN[+1,768.640]NTAVGHALVLPAGR.D | HexNAc(4)Hex(5)Fuc(1) | 1,384.674 | 3 | 4,152.007 | 4,151.996 | 2.5 | Specific | 204.3 |
| R.QLINALQIN[+2,059.735]NTAVGHALVLPAGR.D | HexNAc(4)Hex(5)Fuc(1)NeuAc(1) | 1,111.528 | 4 | 4,443.091 | 4,443.092 | -0.2 | Specific | 312.9 |
| R.QLINALQIN[+2,350.830]NTAVGHALVLPAGR.D | HexNAc(4)Hex(5)Fuc(1)NeuAc(2) | 1,184.303 | 4 | 4,734.190 | 4,734.187 | 0.6 | Specific | 249.2 |
| R.QLINALQIN[+2,372.812]N[+0.984]TAVGHALVLPAG R.D | HexNAc(4)Hex(5)Fuc(1)NeuAc(2)N | 952.437 | 5 | 4,758.155 | 4,757.153 | -0.2 | Specific | 110.6 |
| R.QLINALQIN[+349.137]NTAVGHALVLPAGR.D | HexNAc(1)Fuc(1) | 911.504 | 3 | 2,732.499 | 2,732.494 | 1.7 | Specific | 400.8 |