REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
penta-His primary antibody	QIAGEN	cat#34660; RRID: AB_2619735
Horse radish peroxidase-linked secondary antibody	Thermo Fisher Scientific	cat# 32230; RRID: AB_1965958
FLAG monoclonal antibody	Cell Signaling	3916S; RRID: AB_10694611
Mouse monoclonal anti rat CD4 (clone OX-68) (for AVEXIS prey and bait protein normalization)	Bio-Rad/AbD Serotec	MCA1022R; RRID: AB_567282
Chemicals, Peptides, and Recombinant Proteins
Draxin peptide: GEVMPTLDMALFDWTDYEDLKP	Genscript	hDraxin-22
Nitrocefin CAS 41906-86-9	Merck	Cat# 484400
Deposited Data
Crystal Structure of rDraxin/ rDCCIg1-Ig4 complex	This study	PDB: 5Z5K
Crystal structure of hNetrin-1/hDraxin-22 complex	This study	PDB: 6FKQ
SAXS experimental data	This study	SASBDB: SASDBZ6
Experimental Models: Cell Lines
HEK293T cells	ATCC	CRL-11268
COS Cells	ATCC	CRL-1651
FreeStyle 293-F Cells	Thermo Fisher Scientific	R79007
Oligonucleotides
5′ ATTTA GCGGCCGCC ATGGCAGGGTCCGTC 3′	This study	rat Draxin forward primer signal sequence.
5′ GCCCC GAGCTC GATGTTGATGAAAGATCCC 3′	This study	rat Draxin, reverse primer
5′ TGGAGCCACCCCCAGTTCGAGAAGGGCGGCT CTCTTCATTTTGTGTCTGAACC 3′	This study	rat DCC (residues 39-422) forward primer
5′ TTAAA GGTACC GGCGGCTGGAGCCACCCC CAG 3′	This study	rat DCC reverse primer
5′ AATAT GAGCTC GATGGCAGGCTTGGGG 3′	This study	rat DCC reverse primer
5′ GCGGCCGCCACC ATGGCTGGGCCTGCCATC CACACCGCTC 3′	(Gao et al., 2015)	human Draxin forward primer with signal sequence
5′ GGCGCGCC GACGTTGATGAAGGATCCCT GGTC 3′	(Gao et al., 2015)	human Draxin full length reverse primer
5′ ATAAGAAT GCGGCCGC CATGGGAACCCTC 3′	This study	common primer from pXLG to AVEXIS vector, forward primer
5′ A GGCGCGCC ATGGTGGTGGTGGTGGTG GAGCTC 3′	This study	common primer from pXLG to AVEXIS vector, reverse primer
Recombinant DNA
Plasmid: pXLG-rDraxin	This study	N/A
Plasmid: pXLG-hNetrin-1	(Finci et al., 2014)	N/A
Plasmid: rDCC Ig1-Ig4	(Chen et al., 2013)	N/A
Plasmid: AVEXIS-hDraxin-prey	(Gao et al., 2015)	Addgene #36148
Plasmid: AVEXIS-hDraxin-bait	(Gao et al., 2015)	Addgene #36149
Software and Algorithms
HKL2000	(Otwinowski and Minor, 1997)	N/A
Phaser	(Bunkóczi et al., 2013)	N/A
Coot	(Emsley et al., 2010)	N/A
Phenix	(Afonine et al., 2012)	N/A
Molprobity	(Chen et al., 2010)	N/A
Pymol	The PyMOL Molecular Graphics System, Version 1.7.x Schrödinger, LLC	N/A
ESPRIPT	(Robert and Gouet, 2014)	N/A
MOSFLM	(Battye et al., 2011)	N/A
SCALA	(Evans, 2006)	N/A
MOLREP	(Vagin and Teplyakov, 2010)	N/A
REFMAC5	(Murshudov et al., 2011)	N/A
CCP4 Suite	(Winn et al., 2011)	N/A
Prism	GraphPad	N/A
RADAVER	Franke et al. (2012)	https://www.embl-hamburg.de/biosaxs/download.html
PRIMUS/Qt	Petoukhov et al. (2012)	https://www.embl-hamburg.de/biosaxs/download.html
CRYSOL	Petoukhov et al. (2012)	https://www.embl-hamburg.de/biosaxs/download.html
DAMMIF	Franke and Svergun, 2009	https://www.embl-hamburg.de/biosaxs/download.html
SASVIEW	NSF DANSE	http://www.sasview.org
Other
Mutagenesis kit	Agilent Techologies	#200521
Dulbecco’s modified eagle medium	DMEM, Biochrom	cat# F 0435
L-glutamine	Biochrom	cat# K 0293
fetal-bovine serum	Biochrom	cat# S 0615
Protease Inhibitor Cocktail tablets	cOmplete ULTRA Tablets, Roche	05892791001
Ni-Sepharose Excel resin	GE Healthcare	cat# 17-3712-02
HiLoad 16/60 Superdex 75 column	GE Healthcare	#28-9893-33
Nickel-NTA agarose beads	QIAGEN	Cat No./ID: 30250