REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-HA (Y-11), rabbit polyclonal	Santa Cruz	Cat#sc-805; RRID: AB_631618
Anti HA affinity matrix, Immobilized Anti-HA high-affinity rat monoclonal antibody	Roche	Cat#11 815 016 001
goat anti-rabbit IgG-HRP	Santa Cruz	Cat#sc-2004; RRID: AB_631746
Bacterial and Virus Strains
Endura competent E.Coli cells	Lucigen	Cat#60242-0
Klebsiella pneumoniae non-virulent strain, food source for Paramecium	Gift from Eric Meyer (ENS,Paris)	N/A
Baculovirus	EMBL, Heidelberg	N/A
Chemicals, Peptides, and Recombinant Proteins
Wheat Grass Powder	Pines International,Lawrence,KS	N/A
B-sitosterol	Calbiochem, Millipore	Cat#567152; CAS: 83-46-5
Ribonucleoside vanadyl complexes	Sigma-Aldrich	Cat#R3380
Complete protease inhibitor cocktail	Roche	Cat#11 836 145 001
30% acrylamid:bisacrylamid 19:1	BioRad	Cat#161-0154
Critical Commercial Assays
T4 Polynucleotide kinase	Thermo Fisher Scientific	Cat#EK0032
MEGAscript T7 Transcription Kit	Thermo Fisher Scientific	Cat#AM1333
GoTaq G2 DNA Polymerase	Promega	Cat#M8296
Wizard SV Gel and PCR Clean-Up System	Promega	Cat#A9281
pGem-T Easy Vector System	Promega	Cat#A3600
Deposited Data
All sequencing data of in vitro cleavage assays	This paper	Accession number ENA: PRJEB20111
Experimental Models: Cell Lines
Sf21 cells	EMBL, Heidelberg	N/A
Experimental Models: Organisms/Strains
Paramecium tetraurelia strain 51	Gift from Eric Meyer (ENS,Paris)	N/A
Oligonucleotides
See Table S1 for complete list	This paper, Microsynth, Switzerland	N/A
Recombinant DNA
DCL2-His	This paper	N/A
DCL5-GST	This paper	N/A
DCL3-Ptiwi09 regulatory, FlagHA	This paper	N/A
Bac-to-Bac HT vector kit	Invitrogen	Cat#10584027
pFastBac M30, GST tagged vector	From EMBL protein facility, Heidelberg	N/A
DCL4-Ptiwi09 regulatory, FlagHA	This paper	N/A
Software and Algorithms
Geneious R6.1.6	Geneious	https://www.geneious.com
WebLogo 3	WebLogo, Crooks et al., 2004	http://weblogo.threeplusone.com/