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. 2018 Mar 22;173(1):260–274.e25. doi: 10.1016/j.cell.2018.02.030

Figure S4.

Figure S4

Effects of Estrogen and SERMs on Proteostasis, Related to Figure 4

(A) Compounds structure of estradiol, 17-AAG, GW7604, raloxifene used in experiments reported in Figures 4 and S4.

(B) Scatterplot of protein half-lives (displayed in h) determined by dynamic SILAC in MCF7 cells treated with vehicle or estradiol. The dashed diagonal indicates the equality line.

(C) Scatterplots showing protein fold changes (FC) observed in mature (upper panel) and nascent (lower panel) forms of proteins in MCF-7 cells treated for 24 h with 17-AAG, estradiol, GW7604 or raloxifene relative to vehicle treated cells. Red closed circles indicate statistically significant regulation (p < 0.05) and the dashed diagonal indicates the equality line. N indicates the number of proteins robustly quantified in both replicates.

(D) Scatterplots showing protein fold changes (FC) observed in mature (left panel) and nascent (right panel) forms of proteins in MCF-7 cells treated for 7 h (left panel) or 24 h (right panel) with GW7604 relative to estradiol treated cells. Red closed circles indicate statistically significant regulation (p < 0.05) and the dashed diagonal indicates the equality line. N indicates the number of proteins robustly quantified in both replicates.