REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-CD3	Abcam	Cat# ab8090
anti-CD28	BD Biosciences	Cat# 555725
GREB1	Abcam	Cat# ab72999
GLUL	BD Biosciences	Cat# 610517
HSP70	Cell Signaling	Cat# 4872
α-tubulin	Sigma	Cat# T6793
IRDye800 (anti-mouse)	Li-Cor	Cat# 925-32210
IRDye680 (anti-rabbit)	Li-Cor	Cat# 925-68071
Biological Samples
Human buffy coats (consented donors, IRB approved)	DRK-Blutspendedienst Mannheim	N/A
Chemicals, Peptides, and Recombinant Proteins
17-AAG	LC LABS	Cat# A-6880
I-BET151	GSK compound collection (Dawson et.al. 2011)	CAS: 1300031-49-5
estradiol	Sigma-Aldrich	Cat# E8875; E2758
GW7604	GSK compound collection	CAS: 195611-82-6
raloxifene	GSK compound collection	CAS: 84449-90-1
JQ1	Carbosynth	Cat# FC43273
calyculin A	Apollo Scientific	Cat# BIC1019
sodium orthovanadate	Sigma-Aldrich	Cat# S6508
JQ1-VHL PROTAC	This publication	N/A
JQ1-Az	This publication	N/A
VHL alkyne	This publication	N/A
IBET-151-PROTAC	This publication	N/A
TMT10plex	ThermoFisherScientific	Cat# 90406
Basal RPMI medium	GIBCO	Cat# A24942-01
Dialysed FBS	GIBCO	Cat# 26400-044
Glucose 2g/L	GIBCO	Cat# A24940-01
L-Glutamine (200 mM)	GIBCO	Cat# 24030-032
L-Arginine light	Sigma	Cat# A8094-25G
L-Lysine light	Sigma	Cat# L9037-25G
L-Arginine heavy (SILAC)	Thermo	Cat# 88434
L-Lysine heavy (SILAC)	Sigma	Cat# 608041-1G
Cy3-Oligo(dT)50	Sigma	N/A
Wheat Germ Agglutinin (WGA) Alexa Fluor 647 Conjugate	Life Technologies	Cat# W32466
Hoechst 33258	Sigma	Cat# H3569
Recombinant FYTTD1	Abcam	Cat# ab164533
Protein G beads	Sigma	Cat# P7700
benzonase	Sigma	Cat# E1014
Igepal CA-630	Sigma	Cat# I8896
Critical Commercial Assays
T cells negative selection kit	StemCell Technologies	Cat# 19051
CellTiter-Glo	Promega	Cat# G9242
RNAquous extraction kit	Life Technologies	Cat# Q10212
Maxima First Strand cDNA kit	Thermo Fisher	Cat# K1641
Qubit kit	Life Technologies	Cat# Q10212
TaqMan master mix	Thermo Fisher	Cat# 4369016
Deposited Data
Supplemental datasets for individual Figures	Mendeley Data	https://doi.org/10.17632/8pzhg2tdyb.1
Multiplexed proteome dynamics profiling of JQ1-PROTAC	PRIDE	PXD008637
Thermal proteome profiling of JQ1	PRIDE	PXD008638
2 Dimensional Thermal proteome profiling of JQ1	PRIDE	PXD008626
Multiplexed proteome dynamics profiling of Estradiol and SERMs	PRIDE	PXD008636
Expression proteomics combination treatment of Estradiol and SERMs	PRIDE	PXD008628
Multiplexed proteome dynamics profiling of HSP90 inhibitor 17-AAG	PRIDE	PXD008633
Immunoaffinity enrichment of GREB1 to co-enrich HSP90	PRIDE	PXD008631
Thermal proteome profiling of Jurkat cells	PRIDE	PXD008639
Kinobeads affinity enrichment from lysate derived from stimulated cells	PRIDE	PXD008632
Jurkat protein half live	PRIDE	PXD008629
T cell protein half live	PRIDE	PXD008630
Multiplexed proteome dynamics profiling of HSP90 inhibitor 17-AAG in T cells	PRIDE	PXD008635
Multiplexed proteome dynamics profiling of HSP90 inhibitor 17-AAG in anti-CD3 and anti-CD28 stimulated T cells	PRIDE	PXD008634
Expression profiling of HSP90 inhibitor 17-AAG in anti-CD3 and anti-CD28 stimulated T cells	PRIDE	PXD008627
Experimental Models: Cell Lines
THP-1	ATCC	TIB-202
MCF-7	ATCC	HTB-22
Jurkat	ATCC	TIB-152
Oligonucleotides
Amplicon context sequence for GLUL (FAM)	Thermo Fisher	Cat# 4331182
ACCCGGACCCTGGACA GTGAGCCCAAGTGT GTGGAAGAGT		Assay ID# Hs00365928
Amplicon context sequence for RPL7 (VIC)	Thermo Fisher	Cat# 4331182
AGAACCCAAATTGGC GTTTGTCATCAGAATCAGAGGTA		Assay ID# Hs02596927
Software and Algorithms
ImageJ 1.45 s	NIH	https://imagej.nih.gov/ij/download.html
CellProfiler 2.1.1	Broad Institute	http://cellprofiler.org
Mascot 2.4	Matrix Science, Boston, MA	N/A
isobarQuant	Franken et al., 2015	https://github.com/protcode/isob/archive/1.1.0.zip
R 3.4.1	R Core Team	https://www.R-project.org
TPP library	Franken et al., 2015	http://bioconductor.org/biocLite.R; library(“TPP”)
Spotfire 7.0.1.24	Tibco	https://spotfire.tibco.com
Graphpad Prism 7	GraphPad Software	https://www.graphpad.com