Fig. 7.

AID R-mutants are uncoupled from transcription elongation. a, c–e ChIP qPCR results using anti-AID antibody on extracts from reconstituted Aicda^−/− mouse B cells activated with LPS and IL-4. Means + s.e.m. from two independent experiments are plotted. A scheme of each genomic region analysed is shown at the top of each panel with the qPCR amplicons indicated. b ChIP qPCR for endogenous AID and Spt6 from wt mouse B cells treated and analysed as in a, including amplicons in the non-transcribed Sα region. Means + s.d. from three independent experiments are plotted. f (Bottom) ChIP qPCR for AID from reconstituted DT40 Aicda^−/− ΔΨVλ B cells. Means + s.d. from three independent experiments are plotted. An additional amplicon within Gapdh was used as a negative control. g WB of cell extract from reconstituted Aicda^−/− mouse B cells, probed with antibodies recognizing GFP and AID. For gel source data, see supplementary Fig. 7