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. 2018 Mar 28;8:5328. doi: 10.1038/s41598-018-23741-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of the sr¹ mutation on the expression of pigmentation enzyme coding genes. (a) Ddc expression in thorax of pharates visualized using Ddc-Gal4 and UAS-mCherry-NLS transgenes (UAS-mCherry-NLS/+; Ddc-Gal4). (b) Ddc expression in a sr¹ background (UAS-mCherry-NLS/+; Ddc-Gal4, sr¹/sr¹). (c) ple expression visualized using ple-Gal4 and UAS-mCherry-NLS transgenes (UAS-mCherry-NLS/+; ple-Gal4/+). (d) ple expression in a sr¹ background (UAS-mCherry-NLS/+; ple-Gal4, sr¹/sr¹). (e) t expression followed using the t_MSE-nEGFP transgene. (f) t expression in a sr¹ background (t-MSE-nEGFP; sr¹). (g) e expression visualized using the e-nEGFP transgene. (h) t expression in a sr¹ background (e-nEGFP, sr¹/sr¹). (i) y expression followed using the y-wb-nEGFP transgene. (j) y expression in a sr¹ background (y-wb-nEGFP; sr¹).