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. 2018 Mar 28;8:5333. doi: 10.1038/s41598-018-23250-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell viability and proliferation. (A) MDA-MB-231 cells cultured with materials for 24 h, stained with fuorescence dyes: calceinAM (green) for live cells and propidium iodide (red) for dead cells. (B) Procedure for nuclei segmentation to count cell number within different materials at different time points (C) MDAMB- 231 cell proliferation obtained by nuclei segmentation after 4 and 7 days of culture for different materials. Symbol *indicates statistical significance (ANOVA test; number of samples: N ≥ 10; p < 0.05).