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. 2018 Mar 28;8:5307. doi: 10.1038/s41598-018-23563-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Amplification chemistry and its relationships to (a) sample contamination estimates and (b) variant counts and PC-AF values. All values are plotted separately for each combination of tumour type and amplification chemistry represented in the analysis. The colours distinguish between variants called in “high-contamination” samples (Conpair contamination estimate > = 0.5%) and variants coming from samples with “low contamination” (Conpair contamination estimate < 0.5%). BrAmp: bridge amplification; FL: follicular lymphoma; SARC: sarcoma; DLBCL: diffuse large B-cell lymphoma; SCV: suspected contaminant variant.