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. 2018 Mar 28;8:5344. doi: 10.1038/s41598-018-23625-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Photobleaching kinetic of complemented sGFP variants. (a) Model for irreversible photobleaching (FP_ible) and reversible photoconvertible dark state reactions (FP_rble) for a native GFP (FP_nat). (b) Normalized photobleaching kinetics for complemented sGFPori, sGFP1, sGFP2 and sGFP3. (c) Comparison of the forward (k₁, left panel) and backward (k₂, middle panel) photoconvertible dark state rate constants and of the irreversible photobleaching rate constants (k₃, right panel) for all complemented sGFP variants.