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. 2018 Mar 28;8:5339. doi: 10.1038/s41598-018-23636-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of silique phenotype between multilocular and bilocular plants in BC3F5 generation. (a) An anatomical diagram of a bilocular and a multilocular silique in BC3F5 plants. (1–2) A normal B. juncea silique with two valves (2), or with both the valves removed (1), and two rows of seeds in it. (3–4) A multilocular silique with four valves (4), or the four valves have been removed (3), and four rows of seeds in it. (5–6) A multilocular silique possessing an additional pistil in the silique (6), an additional gynoecium inside the silique that has been peeled, and seeds are in and around the gynoecium (5). (b) Cross-sections of the gynoecium from the bilocular and multilocular plants at different stages of development. (1) Bilocular plant of BC3F5, stages 7–9. (2) Bilocular plant of BC3F5, stages 10–11. (3) Multilocular plant of BC3F5, stages 7–9. (4) Multilocular plant of BC3F5, stages 10–11. M, medial region; L, lateral region; O, ovule; Lc, locule.