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. 2018 Mar 28;8:5339. doi: 10.1038/s41598-018-23636-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression pattern analysis of BjuA07.CLV1 between bilocular and multilocular plants of near isogenic line using real time quantitative polymerase chain reaction (RT-qPCR) and β-glucuronidase (GUS) assay. (a) Comparison of BjuA07.CLV1 expression between NIL (BL) and NIL (ML) in various tissues by using qRT-PCR. The reference gene is β-actin. The values are the averages of three biological replicates; SDs are shown as error bars. **P < 0.01, and *P < 0.05. (b) GUS expression patterns (blue staining) in different tissues from the Pro_BjuA07._CLV1-GUS transgenic line; With the exception of siliques (4), GUS staining was detected in all tested tissues, including inflorescence (1), cotyledon (2), seedling (3), root (2,3), stem (5), bud (6), SAM (7) and flower (8).