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. 2018 Mar 28;8:5339. doi: 10.1038/s41598-018-23636-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sequence analysis of BjuA07.CLV1 and its subcellular localisation. (a) MEGA4.0 was used for phylogenetic tree construction using the bootstrap neighbour-joining method. Proteins were designated according to the gene names of the accession numbers in NCBI. The branch lengths show variation rates of the amino acids. (b) Subcellular localisation of the BjuA07.CLV1-GFP fusion protein in Arabidopsis protoplasts. (1) The protoplast cell with BjuA07.CLV1-GFP fusion construct showed green fluorescent signal under a fluorescent filter; (2) The same cell under bright field; (3) The images in (1) and (2) were merged. Bars = 10 μM in (1–3); (4) The green fluorescent signal was detected throughout the protoplast cells with control GFP construct; (5) The same cell as (4) under bright field; (6) The images in (4) and (5) were merged. Bars = 10 μM in (4–6).