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. 2018 Mar 21;9:260. doi: 10.3389/fphys.2018.00260

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Copyright © 2018 Fung, Koussoulas, Unterweger, Allen, Bornstein and Foong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Myenteric neurons of CT-incubated ileal segments displayed increased spontaneous firing. Representative micrographs showing myenteric neurons that were injected with biocytin (axons indicated by arrows) during recording and their corresponding intracellular recordings from control (A) and CT-treated (B) preparations. Myenteric neurons showed an increase in spontaneous activity (single and bursts of fast EPSPs indicated by arrow heads) following CT exposure. (C) Graphical representation of the significantly higher proportion of myenteric neurons that displayed spontaneous firing of fast EPSPs after CT-treatment. Numbers of spontaneously active neurons are displayed within each histogram (^**P < 0.01).