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. 2017 Jul 25;1:24. doi: 10.1038/s41698-017-0028-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — KRAS mutation detection. a Schematic of the polymerase chain reaction/ligase detection reaction (PCR/LDR) assay. b Electropherograms of LDR products for: No gDNA; HT29 wt35 (50 nt); LS180 G35A (44 nt); M-PDAC CTC^FAPα G35A (44 nt), CTC^EpCAM G34C (61 nt); and CTC^FAPα G35T (55 nt). The gray trace shows the DNA markers. The fluorescence intensity values are arbitrary. c Table summarizing PCR/LDR results for HT29 and LS180 cell lines, M-CRC, L-CRC, M-PDAC, and L-PDAC CTCs. d RT-qPCR gene expression profiles for L-PDAC patient #66 and M-PDAC patient #67