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Primer combinations used in adaptive mutation plasmid construction
| Fragment | Template |
Primers |
|
| Sense | Anti-sense | ||
| mE2-1 | JFH1 | 1340-F | m2310-R |
| mE2-2 | m2310-F | 3500-R | |
| mp7-1 | 1340-F | m2681-R | |
| mp7-2 | m2681-F | 3500-R | |
| mNS4B-1 | 5249-F | m6132-R | |
| mNS4B-2 | m6132-F | 7791-R | |
| mNS5A-1 | 5249-F | m7160-R | |
| mNS5A-2 | m7160-F | m7359-R | |
| mNS5A-3 | m7359-F | m7658-R | |
| mNS5A-4 | m7658-F | 7966-R | |
| mNS4B/NS5A-1 | JFH1-mNS5A | 5249-F | m6132-R |
| mNS4B/NS5A-2 | m6132-F | 7791-R | |
| mE2/p7 -1 | JFH1-mp7/NS5A | 1350-F | m2310-R |
| mE2/p7 -2 | m2310-F | 3500-R | |
